Supply of Laboratory Equipment (Part CIX) for SES of the Republic of Uzbekistan.

New amendment added #2: The ITB has been further amended to reflect the changes for technical specifications for Lot#1, Lot#3, Lot#4 and Lot#5  the document title "Section_III_Returnable_Bidding_Forms_Form D_Technical Bid Form" is hereby replaced with the updated documents titled "Section_III_Returnable_Bidding_Forms_Form D_Technical Bid Form Rev 1".All other requirements, terms and conditions remain unchanged.

New clarification added: Q79. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section G - Mass spectrometer, Line 18 (Mass range: not narrower than 50–2000 m/z), corresponds a specific equipment by a single manufacturer, please kindly revise this requirement to encourage more participation.A79. The requirement “Mass range: not narrower than 50–2000 m/z” defines the minimum operational capability required for the mass spectrometer to perform the intended analytical tasks, including the determination of antibiotic residues in food matrices.It should be noted that this requirement specifies a minimum acceptable mass range. Systems offering a wider operational mass range remain fully compliant with the specification.Therefore, LC-MS/MS systems with a mass range such as 40–2000 m/z or wider will be considered fully compliant, provided that all other technical requirements of the specification are met.The requirement “Mass range: not narrower than 50–2000 m/z” remains unchanged.

New clarification added: Q78. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section F - Column thermostat, Line 15 (Temperature stability is no worse than ±0.1 °C), it favors a single manufacturer, so please increase temperature stability to ±0.3 °C, for example, to encourage more participation.A78. Column temperature stability is an important parameter for maintaining reproducible chromatographic retention times and ensuring consistent analytical performance during liquid chromatography analysis. Stable temperature control contributes to reproducible chromatographic separation and reliable quantification of analytes, particularly in multi-residue analytical workflows.At the same time, it is acknowledged that different manufacturers specify column thermostat stability using slightly different values and measurement methodologies. Modern LC systems commonly provide temperature stability in the range of ±0.1 °C to ±0.3 °C, and both ranges are generally sufficient for typical LC-MS analytical applications, including the determination of antibiotic residues in food matrices.Considering the intended analytical application and the fact that chromatographic methods for veterinary drug residues are typically performed at fixed column temperatures, a minor difference in temperature stability does not materially affect analytical performance.In order to avoid unnecessarily restricting participation of technically suitable systems while maintaining reliable chromatographic performance, the requirement will be revised as follows:Revised requirement – Item 15Column thermostat temperature stability: not worse than ±0.3 °C.

New clarification added: Q77. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section G - Mass spectrometer, Line 25 (The MRM transition registration speed is at least 500 transitions/s), yet for LC-MS/MS analysis of antibiotic residues in milk and meat, typical methods use far fewer transitions (around 50–100), which is sufficient for multiresidue analysis. Please revise this requirement to encourage more participation.A77. Thank you for your comment regarding Lot #3 – LC-MS/MS, specifically Item 25 concerning the MRM transition registration speed.The parameter specifying the MRM transition acquisition speed reflects the capability of the mass spectrometer to monitor multiple ion transitions within a single chromatographic run. A higher transition acquisition speed allows the instrument to monitor a larger number of compounds simultaneously while maintaining sufficient data points across chromatographic peaks, which is important for reliable quantification and confirmation of analytes in multi-residue analytical methods.While some analytical methods for antibiotic residue determination may involve a smaller number of transitions, modern multiresidue screening workflows frequently include a large number of compounds and corresponding confirmation transitions, which may significantly increase the total number of monitored transitions within a single analytical run.It should also be noted that LC-MS/MS systems from several major manufacturers are capable of achieving MRM acquisition speeds at or above the specified level.Therefore, the requirement reflects a performance capability of the instrument rather than a mandatory operational condition, ensuring flexibility for present and future analytical methods.The requirement “MRM transition registration speed: at least 500 transitions/s” remains unchanged.This parameter ensures sufficient analytical flexibility while remaining achievable for modern LC-MS/MS systems from multiple manufacturers.

New clarification added: Q76. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section F - Column thermostat, Line 14 (Temperature range: from (ambient temperature -10 °C) to 85 °C), the technical specification does not provide an analytical or methodological justification for establishing this precise limit and correspond to the parameter of equipment from a single manufacturer. Please revise this requirement to encourage more participation.A76. Thank you for your comment regarding Lot #3 – Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS), specifically Item 14 concerning the temperature range of the column thermostat.The specified temperature range was originally included to ensure sufficient operational flexibility for chromatographic separations used in the analysis of antibiotic residues and other analytes in food matrices. Elevated column temperatures may improve chromatographic efficiency, reduce backpressure, and enhance peak shape for certain compounds.At the same time, it is acknowledged that different manufacturers express the operating temperature range of column thermostats using slightly different absolute values or reference conditions. Modern UHPLC column ovens from several manufacturers provide operating ranges suitable for LC-MS analytical applications even when the maximum temperature differs slightly from the originally specified value.In order to avoid unnecessarily limiting participation of technically suitable systems while maintaining the required analytical functionality, the requirement will be clarified as follows:Revised requirement – Item 14Column thermostat temperature range: from at least 20 °C up to at least 80 °C.This temperature range remains fully sufficient for typical UHPLC and LC-MS analytical workflows and allows participation of systems from multiple manufacturers.

New clarification added: Q75. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section C - Liquid chromatograph (UHPLC), Line 20 ("Ionization source:- electrospray (ESI) with the ability to operate in positive and negative modes;- Polarity switching time is no more than 5 ms), and Line 21 (Sensitivity is estimated as a signal-to-noise ratio (S/N) ≥ 850,000 when administering 1 pg reserpine or equivalent compound), polarity switching speed: 5 ms and Signal-to-noise ratio (S/N): 850,000. These specifications describe the required instrument performance parameters for the LC-MS/MS system used for the analysis tasks mentioned in Clauses 1 and 3 (antibiotic residue analysis in milk and meat). However, instruments with less stringent parameters (polarity switching speed around 15–20 ms and lower S/N values) are widely used in international analytical practice while still providing the required sensitivity and reliability of results. Please kindly revise these requirements to encourage more participation.A75. Thank you for your comment regarding Lot #3 – LC-MS/MS, specifically the requirements related to polarity switching time and system sensitivity.The requirement for electrospray ionization (ESI) capable of operating in both positive and negative modes reflects the analytical needs of the intended application, namely the quantitative and confirmatory determination of antibiotic residues in food matrices such as milk and meat. Many compounds included in multi-residue veterinary drug methods ionize preferentially in different ionization modes, and the capability to operate in both modes is therefore necessary for the intended analytical workflows.Item 20 – Polarity switching timeIonization polarity switching time is a design characteristic that may vary between manufacturers depending on the architecture of the ion source and the mass spectrometer electronics. While rapid switching between positive and negative ion modes may improve operational convenience in certain analytical methods, it does not represent a fundamental analytical capability required for the intended applications described in the specification.In order to avoid restricting participation of technically suitable LC-MS/MS systems using different source architectures, the polarity switching time parameter will be removed from the specification.Item 21 – SensitivityThe specified sensitivity value represents a target performance level intended to ensure reliable detection of analytes at trace concentrations during the analysis of antibiotic residues in complex food matrices.It is acknowledged that manufacturers may report sensitivity using slightly different reference compounds, measurement conditions, or calculation methodologies.

New clarification added: Q74. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section C - Liquid chromatograph (UHPLC), Line 26 (Availability of specialized client-server software developed by the manufacturer of chromatographic equipment) and Line 27 (The client-server software environment must ensure the legitimacy, originality, accuracy, and integrity of electronic records, centralized license management, connection of chromatographic and chromatographic-mass-spectrometric equipment from different manufacturers, data storage, user accounts, automatic archiving schedules, protection, recovery, and preservation of data in normal and abnormal operating modes), yet proprietary software developed by instrument manufacturers is generally intended to operate hardware produced by the same manufacturer and typically does not support full control of mass spectrometry systems from other vendors. Please clarify these requirements as they contradict each other.A74. Thank you for your comment regarding the software requirements specified in Lot #3 – LC-MS/MS, particularly Item 26 (Availability of specialized client-server software developed by the manufacturer of chromatographic equipment) and Item 27 (Client-server software environment functionality), as well as the workstation configuration.The purpose of these requirements is to ensure that the proposed LC-MS/MS system includes a reliable software environment capable of supporting instrument control, analytical data acquisition, processing, and secure management of electronic records generated during chromatographic and mass spectrometric analysis.The requirement for specialized client-server software developed or officially supported by the manufacturer refers to the standard instrument control and data processing platform supplied with the chromatographic and mass spectrometric system. Such software is typically designed to control the instrument modules of the system and manage analytical data generated during operation.The functions described in Item 27 relate to the data management and security capabilities of the software environment. These functions include, but are not limited to:management of user accounts and access rights,protection and integrity of electronic records,audit trail functionality,centralized license management,data storage and archiving,automatic backup scheduling,protection, recovery, and preservation of analytical data in normal and abnormal operating modes.It is acknowledged that proprietary software supplied by instrument manufacturers is generally optimized for operation of equipment produced by the same manufacturer and may not provide direct operational control of analytical instruments from other vendors.Therefore, the requirement regarding connection of chromatographic and chromatographic-mass-spectrometric equipment from different manufacturers should be interpreted as the capability of the software environment to support integration, compatibility, or data exchange with other laboratory information systems or analytical platforms where applicable, rather than requiring direct hardware control of instruments produced by other manufacturers.

New clarification added: Q73. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section C - Liquid chromatograph (UHPLC), Line 20 ("Ionization source:- electrospray (ESI) with the ability to operate in positive and negative modes;- Polarity switching time is no more than 5 ms), and Line 21 (Sensitivity is estimated as a signal-to-noise ratio (S/N) ≥ 850,000 when administering 1 pg reserpine or equivalent compound), polarity switching speed: 5 ms and Signal-to-noise ratio (S/N): 850,000. These specifications describe the required instrument performance parameters for the LC-MS/MS system used for the analysis tasks mentioned in Clauses 1 and 3 (antibiotic residue analysis in milk and meat). However, instruments with less stringent parameters (polarity switching speed around 15–20 ms and lower S/N values) are widely used in international analytical practice while still providing the required sensitivity and reliability of results.A73. Thank you for your comment regarding Lot #3 – Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS), specifically the parameters related to ionization source switching time and system sensitivity.Item 20 – Ionization source / polarity switchingIonization source switching time is a design characteristic that may vary between manufacturers depending on the ion source architecture. While rapid switching between positive and negative ionization modes may improve operational convenience in certain analytical workflows, it does not significantly affect the analytical capability of the system for the intended applications described in the technical specification.In order to avoid restricting participation of technically suitable LC-MS/MS systems using different source configurations, this parameter will be removed from the specification.Item 21 – SensitivityThe specified sensitivity value represents a target performance level intended to ensure reliable detection of analytes at trace concentrations during the analysis of antibiotic residues in food matrices.However, it is acknowledged that manufacturers may report sensitivity using slightly different measurement conditions or reference compounds. Therefore, minor deviations within standard industry specifications, supported by official manufacturer documentation and demonstrating equivalent analytical sensitivity suitable for trace-level analysis, may be considered acceptable during technical evaluation.

New clarification added: Q72. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section D - Components, spare parts, consumables, Line 32 (Ion sources included as standard with switching time not more than 20 ms), please confirm if the requirement should be interpreted as follows: 'Ion sources included as standard with switching time not more than 20 ms.' or whether this parameter refers to the polarity switching time between positive and negative ionization modes (ESI+/ESI−) during data acquisition, as in mass spectrometry the term “switching time” typically applies to polarity switching rather than to ion sources.A72. "Ionization source switching time is a design characteristic that may vary between manufacturers depending on the source architecture. While rapid switching may improve operational convenience, it does not significantly affect the analytical capability of the system for the intended applications.In order to avoid restricting participation of technically suitable systems using different source configurations, this parameter will be removed.

New clarification added: Q71. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 19 (Heating rate: from no more than –250 to no less than +250 °C/min), the published tender specifications appear to align closely with the technical characteristics of a single model and may therefore require review to ensure compliance with local regulations and to maintain open and fair competition so please kindly change it to 'Heating rate: not less than 150°C/min'.A71. Thank you for your comment regarding Lot 5 – GC with FID and MSD, specifically Section E – Flame Ionization Detector (FID), Item 19 (Heating rate).In order to avoid unnecessarily limiting participation while maintaining the required analytical performance, the requirement will be clarified as follows:Revised requirement (Item 19):Heating rate: not less than 150 °C/min.This level of heating performance remains sufficient for typical GC analytical methods while allowing broader participation of systems from different manufacturers.Please note that the specification is intended to remain technology-neutral and not linked to any specific manufacturer or model.

New clarification added: Q70. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 18 (Temperature programming: at least 32 stages, with programmable cooling option), the published tender specifications appear to align closely with the technical characteristics of a single model and may therefore require review to ensure compliance with local regulations and to maintain open and fair competition so please kindly change it to 'Temperature program ramps/holds: 24/25'.A70. The requirement “Temperature programming: at least 32 stages, with programmable cooling option” was originally included to ensure sufficient flexibility for complex chromatographic methods used in the analysis of fatty acid methyl esters (FAMEs), cis/trans isomer separation, and derivatized amino acid analysis.However, after reviewing your comment and considering the principle of fair competition and participation of multiple manufacturers, we acknowledge that modern gas chromatography systems from several manufacturers provide comparable analytical capabilities with different numbers of programmable temperature ramps/holds.In order to avoid unnecessarily limiting participation while maintaining the required analytical performance of the system, the requirement will be clarified as follows:Revised requirement (Item 18):Temperature programming capability with at least 24 programmable ramps/holds or equivalent functionality enabling complex temperature programs required for chromatographic analysis.This clarification ensures that systems from multiple manufacturers can participate while preserving the required analytical functionality of the gas chromatograph.Please note that the specifications are intended to remain technology-neutral and not linked to any specific manufacturer or model, and the tender continues to allow equivalent systems meeting the functional requirements.

New clarification added: Q67. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section D - Components, spare parts, consumables, Line 108 (The supplier must provide the laboratory with a validated analytical method for the following type of analysis: 1. Pesticides. 2. Antibiotics. 3. Dyes.), as universally validated methods for all these classes do not exist please kindly change it to 'The supplier must provide the laboratory with recommended or pre-validated analytical procedures, which can be further validated by the laboratory for specific applications' to encourage more participation.A67. The purpose of this requirement is to ensure that the laboratory receives sufficient methodological support for the implementation of the LC-MS system in routine analytical work. However, it is acknowledged that analytical methods for complex matrices such as food products typically require laboratory-specific validation in accordance with applicable regulatory or accreditation standards.Manufacturers and suppliers of LC-MS systems commonly provide recommended analytical workflows, application notes, and pre-validated procedures, which serve as a starting point for laboratories to adapt and validate methods under their own operating conditions.In order to ensure practical implementation of the system for universally validated methods across all compound classes, the requirement will be revised.Revised requirement:The supplier must provide the laboratory with recommended or pre-validated analytical procedures for the determination of pesticides, antibiotics, and dyes, which can be further validated by the laboratory for specific applications.

New clarification added: Q66. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section D - Components, spare parts, consumables, Line 73 (Four-channel pump with integrated degasser – 1 set), as an integrated degasser is not mandatory, please kindly remove this requirement to encourage more participation.A66. Degassing of mobile phases is an important element of liquid chromatography systems as it helps prevent the formation of gas bubbles in the solvent lines and pump heads, which can affect pump stability, flow accuracy, and chromatographic reproducibility.Modern LC systems typically use either integrated vacuum degassers within the pump module or separate degasser modules installed in the solvent flow path. Both configurations are widely used by leading manufacturers and provide equivalent functionality for removing dissolved gases from mobile phases.In order to ensure reliable operation of the LC system while allowing broader participation of technically suitable instruments, the requirement will be clarified to focus on the functional presence of a degassing system rather than its structural implementation.Revised requirement:Four-channel pump with degassing system (integrated or external) – 1 set.

New clarification added: Q65. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section D - Components, spare parts, consumables, Line 72 (Combined ESI/APCI ion source – 1 pc.), as combined ion sources are generally less efficient, please kindly chage it to 'Ionization sources — ESI and APCI (one of each), included as standard' to encourage more participation.A65. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) are widely used and complementary ionization techniques in LC-MS analysis. Depending on the chemical properties of analytes, either ionization mode may be required for optimal sensitivity and analytical performance.It is acknowledged that manufacturers implement these ionization techniques using different technical designs. Some LC-MS systems provide combined ESI/APCI ion sources, while others supply separate interchangeable ESI and APCI sources as standard modules.Both approaches can provide the required analytical capability for the intended applications, including the determination of antibiotics, dyes, and other compounds in food matrices.In order to ensure broader participation of technically suitable LC-MS systems while maintaining the availability of both ionization techniques, the requirement will be clarified.Revised requirement:Ionization sources: ESI and APCI available, either as a combined source or as separate modules supplied with the system.

New clarification added: Q64. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 41 (Quadrupole isolation range: At least 3800 m/z.), a strict limit is not justified, please kindly change it to 'Quadrupole isolation mass range — at least 2000 m/z or according to the manufacturer’s specification'.A64. The quadrupole isolation range defines the mass-to-charge (m/z) values that can be selected and transmitted by the quadrupole prior to high-resolution analysis in the time-of-flight analyzer. This parameter is important for enabling precursor ion selection during MS/MS experiments.However, for the intended analytical applications—namely the determination of antibiotics and dyes in food raw materials and food products using LC-MS with high-resolution mass spectrometry—the relevant analytes typically fall within significantly lower mass ranges.In order to ensure adequate analytical capability while allowing broader participation of systems from multiple manufacturers, the requirement will be revised.Revised requirement:Quadrupole isolation range: at least 2000 m/z.

New clarification added: Q63. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 34 (At least 4 integrated sample/syringe reservoirs for automated system setup/calibration controlled by software), the specification “sample/syringe” restricts competition, please kindly remove this requirement to encourage more participation.A63. The purpose of this requirement is to ensure that the mass spectrometer system supports automated setup, tuning, and calibration procedures controlled through the system software, using dedicated reservoirs for calibration solutions and related system fluids.It is acknowledged that different manufacturers implement automated calibration and system setup using different technical approaches and terminology. Some systems utilize dedicated calibration solution reservoirs, while others may integrate calibration fluids through alternative fluid delivery mechanisms.In order to ensure broader participation of technically suitable high-resolution LC-MS systems while maintaining the required capability for automated system setup and calibration, the wording of the requirement will be revised to focus on the functional capability rather than the specific fluid delivery configuration.Revised requirement:At least 4 integrated reservoirs for automated system setup and calibration controlled by system software.

New clarification added: Q62. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 32 (Ion sources included as standard with switching time not more than 20 ms.), as the Ion source switching time is not an essential criterion, please kindly remove this requirement to encourage more participation.A62. Ionization source switching time is a design characteristic that may vary between manufacturers depending on the source architecture. While rapid switching may improve operational convenience, it does not significantly affect the analytical capability of the system for the intended applications.In order to avoid restricting participation of technically suitable systems using different source configurations, this parameter will be removed.

New clarification added: Q61. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 30 (Mass analyzer: Benchtop hybrid quadrupole time-of-flight (Q-TOF) mass spectrometer), please change it to 'Benchtop or floor-standing hybrid quadrupole time-of-flight (Q-TOF) mass spectrometer'.A61. The system is intended for the determination and development of analytical methods for antibiotics and dyes in food raw materials and food products using high-performance liquid chromatography coupled with high-resolution mass spectrometry.The original specification referred specifically to a benchtop Q-TOF configuration, which is commonly used in LC-MS laboratories. However, the physical configuration of the mass spectrometer (benchtop or floor-standing) does not affect the analytical performance of the system, provided that all required technical parameters for high-resolution LC-MS analysis are met.Furthermore, high-resolution LC-MS systems used for food safety analysis may be implemented using different high-resolution mass analyzer technologies offered by various manufacturers.To ensure adequate analytical performance while allowing broader participation of suitable high-resolution LC-MS systems, the specification will be revised.Revised wording for Item 30: Benchtop or floor-standing hybrid quadrupole time-of-flight (Q-TOF) or equivalent high-resolution mass spectrometer.

New clarification added: Q60. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 27 (Precision: < 0.25%), as the parameter is not mandatory for autosamplers, please kindly remove it to encourage more participation.A60. Injection precision is an important parameter influencing chromatographic reproducibility and quantitative accuracy.However, different manufacturers express autosampler precision using different definitions (e.g., injection repeatability, peak area precision, or volume precision), which may lead to ambiguity during evaluation.To avoid potential misinterpretation of manufacturer specifications while maintaining the required analytical performance, this parameter will be removed.Result: The requirement is removed from the specification.

New clarification added: Q59. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 26 (Sample temperature control: At least 4°C to 40°C in 0.1°C increments), as 0.1 °C increment is not critical, please change it to 'Recommendation: “Sample temperature control: At least 4 °C to 40 °C.' to encourage more participation.A59. The sample temperature control function is important for maintaining stability of thermally sensitive compounds during autosampler storage.The specified temperature range reflects standard performance of modern LC autosamplers. However, the exact temperature adjustment step does not significantly affect analytical performance.Revised requirement: Sample temperature control: at least 4 °C to 40 °C.

New clarification added: Q58. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 25 (Injection volume range: At least 0.1 to 1000 µL), as for MS/MS and Q-TOF systems, smaller injection volumes are typically used, please change it to 'Injection volume range — at least 0.1–50 µL' to encourage more participation.A58. Clarification Answer: the originally specified injection volume range was intended to provide flexibility for a wide range of analytical workflows, including sample preparation procedures that may require larger injection volumes.However, for LC-MS and high-resolution Q-TOF systems used in food safety analysis, injection volumes are typically significantly smaller in order to maintain chromatographic peak shape and avoid detector saturation. In order to ensure broader participation of technically suitable LC systems while maintaining practical analytical capability, the requirement will be revised.Revised requirement: Injection volume range: at least 0.1–50 µL.

New clarification added: Q57. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 22 (Temperature range: 20-90°C, adjustable in 0.1°C increments), the standard temperature range of leading manufacturers is wider. Please change it to 'Temperature range — from at least (ambient −10 °C) to 80 °C' to encourage more participation.A57. The specified temperature range was defined to ensure sufficient operational flexibility for chromatographic separations used in food safety analysis. Elevated column temperatures may improve peak shape, reduce backpressure, and enhance chromatographic efficiency for certain compounds.Modern LC column thermostats from major manufacturers are capable of operating across wide temperature ranges suitable for UHPLC applications.However, it is acknowledged that manufacturers may express the lower limit relative to ambient temperature rather than as an absolute value.Revised requirement:Temperature range: from at least 20°C up to at least 80 °C.

New clarification added: Q56. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 19 ("Column Thermostat.Capacity: At least 2 columns up to 15 cm long in separate compartments, allowing for different temperatures."), this requirement “in separate compartments” restricts competition.Please keep the column capacity requirement without specifying structural design details.A56. The purpose of this requirement is to ensure operational flexibility by enabling installation of multiple analytical columns within the column thermostat and allowing efficient switching between chromatographic methods without frequent manual replacement of columns.It is acknowledged that different LC system manufacturers implement column oven designs using various technical solutions. Some systems provide separate temperature-controlled compartments, while others allow installation of multiple columns within a single temperature-controlled compartment combined with column switching valves.In order to ensure broader participation of technically suitable LC systems while maintaining the intended operational functionality, the requirement will be revised.Revised requirement:Column Thermostat capable of accommodating at least two columns up to 15 cm in length with column switching capability.

New clarification added: Q55. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 18 (Total system bandwidth: Max 12 µL.), the parameter is not standardised. Please kindly remove it to encourage more participation.A55. Total system bandwidth is a chromatographic performance parameter describing the effective dispersion of the chromatographic system outside the analytical column. This parameter is related to extra-column volume and dispersion, which may influence peak broadening, chromatographic resolution, and sensitivity, particularly when using columns with small particle sizes and narrow peak widths typical for modern LC-MS applications.Although different manufacturers may express this characteristic using different terminology (e.g., extra-column volume, system dispersion, or delay volume), modern high-performance LC systems are designed with optimized flow paths and minimized internal volumes in order to maintain narrow peak dispersion and ensure high chromatographic efficiency.Systems from major manufacturers incorporate low-dispersion flow paths and optimized detector and tubing configurations in order to maintain minimal extra-column dispersion suitable for UHPLC and LC-MS analytical workflows.Therefore, the parameter reflects a performance characteristic related to chromatographic efficiency and sensitivity and is considered technically justified.The requirement “Total system bandwidth: max 12 µL” remains unchanged. 

New clarification added: Q54. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 17 (Gradient delay volume: Not more than 300 µL.), the parametre is not mandatory. Please kindly remove it to encourage more participation.A54. Gradient delay volume (also commonly referred to as dwell volume) is a recognized technical parameter of liquid chromatography systems operating in gradient mode. It represents the internal volume between the point of solvent mixing and the column inlet and determines the time required for the programmed gradient composition to reach the column.Control of gradient delay volume is important for ensuring accurate gradient formation, reproducible retention times, and consistent chromatographic performance, particularly in LC-MS analytical workflows where solvent composition at the column inlet directly affects analyte separation and ionization stability.Although different manufacturers may use the terms gradient delay volume, dwell volume, or system volume, they refer to the same physical parameter affecting gradient performance.Therefore, the specified parameter reflects a standard and well-recognized performance characteristic of modern LC systems and does not restrict participation of systems from major manufacturers.Result: The requirement “Gradient delay volume: not more than 300 µL” remains unchanged.

New clarification added: Q53. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 16 (Composition accuracy: <0.15% RSD), the parameter is not standardised; different manufacturers use different normalisation methods. Please change it to 'Gradient composition accuracy — not more than ±0.5%' to encourage more participation.A53. Gradient composition accuracy is an important performance parameter of LC pumps used in gradient elution, as it ensures precise mixing of solvents and contributes to stable chromatographic separation and reproducible analytical results. Accurate gradient formation is particularly important in LC-MS analytical workflows where solvent composition directly influences chromatographic retention behavior and ionization conditions.It is acknowledged that different manufacturers may express gradient composition accuracy using different reporting formats or calculation approaches. However, modern LC systems from leading manufacturers are capable of achieving gradient composition accuracy within the originally specified performance range.Therefore, the requirement reflects a technically justified performance level for modern LC systems and does not restrict market competition.Result:The requirement “Composition accuracy: < 0.15% RSD” remains unchanged.

New clarification added: Q52. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 15 (Flow accuracy: 0.075% RSD.), please change it to 'Flow accuracy: not less than 0.075% RSD' as some systems have better flow accuracy and also to encourage more participation.A52. Flow accuracy is a key operational parameter of liquid chromatography pumps and directly affects the precision of solvent delivery, gradient composition stability, and chromatographic retention time reproducibility. Accurate flow control is particularly important in LC-MS analytical systems where consistent solvent delivery contributes to stable ionization conditions and reliable analytical results.Modern LC pumps from major manufacturers typically achieve flow accuracy and precision at this level or better. Therefore, the requirement reflects a commonly achievable performance parameter for modern LC systems and does not restrict market competition.To improve clarity of interpretation during evaluation while maintaining the same technical performance level, the wording of the requirement is clarified.Clarified requirement:Flow accuracy: not worse than 0.075% RSD.

New clarification added: Q51. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 14 (Pressure pulsation: ≤1.0%), the parameter is a design characteristic of the pump of one manufacturer. Please remove this requirement to encourage more participation.A51. Pressure pulsation is a recognized operational performance parameter of liquid chromatography pumps and plays an important role in ensuring stable solvent delivery, reproducible chromatographic retention times, and consistent detector response, which are essential for high-precision LC-MS analysis. Excessive pulsation may negatively affect baseline stability, peak shape, and quantitative accuracy, particularly in gradient elution and trace-level analytical applications.It should be noted that major LC-MS system manufacturers offer LC pump modules within their standard product portfolios that meet or exceed this performance level. The requirement therefore reflects a commonly achievable technical performance parameter for modern high-performance LC systems and is not considered to restrict competition.In view of the above, the requirement is considered technically justified and aligned with the intended analytical performance of the system.The requirement “Pressure pulsation ≤ 1.0%” remains unchanged.

New clarification added: Q50. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 12 (Flow rate: 0.001 to 2.000 mL/min with 0.001 mL increments) and limiting the flow rate range reduces functionality. Please change it to 'Flow rate should be from 0.001 to 10 mL/min' without specifying increments to encourage more participation.A50. The specified flow range reflects typical operating conditions for LC-MS analytical systems where lower flow rates are commonly used to optimize chromatographic separation and ionization efficiency.Increasing the maximum flow rate does not provide additional analytical benefit for the intended application.Therefore, the requirement remains unchanged.

New clarification added: Q49. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 11 (Equipped with an integrated active pump inlet valve), the requirement relates to the pump design of a single manufacturer and is not mandatory for ensuring flow stability and analytical accuracy. Please change it to 'equipped with an inlet valve (active or passive type', to encourage more paticipation.A49. Different LC pump architectures may use either active or passive inlet valves to regulate solvent intake. Both configurations are capable of providing stable solvent delivery and reliable chromatographic performance.In order to avoid restricting participation of systems using different pump designs, the requirement is clarified as follows:Revised requirement:Pump equipped with an inlet valve (active or passive type) capable of maintaining stable solvent delivery.

New clarification added: Q48. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical Specifications, Line 4 (Benchtop tandem quadrupole mass spectrometer system), Designation “benchtop” refers only to the structural design and does not affect the analytical characteristics of the system. A floor-standing design does not degrade the functional parameters. Please change it to 'Benchtop or floor-standing tandem quadrupole mass spectrometer system.' to encourage more participation.A48. The specification was developed to reflect the configuration commonly used in LC-MS analytical laboratories. However, the physical configuration itself does not affect analytical performance provided that all required analytical and technical parameters are fully met.In order to allow participation of technically equivalent systems from different manufacturers, the requirement is clarified as follows:Revised requirement:Benchtop or floor-standing tandem quadrupole mass spectrometer system.

New clarification added: Q47.As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 27 (Carrier gas flow rate through the injector: from 0 to at least 1300 ml/min), please change it to 'Carrier gas flow rate through the injector: from 0 to at least 1250 ml/min' to encourage more participation.A47. The carrier gas flow rate parameter defines the maximum flow capability of the injector system. This value represents the operational capacity of the gas chromatograph injector rather than typical flow rates used during capillary gas chromatographic analysis.To allow broader participation of suitable GC systems while maintaining adequate injector performance, the specification will be revised.Original requirementCarrier gas flow rate through the injector: from 0 to at least 1300 ml/min.Revised requirementCarrier gas flow rate through the injector: from 0 to at least 1250 ml/min.

New clarification added: Q46.As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 21 (Thermostat capacity: not less than 13.7 liters), please kindly remove this requirement as it duplicates Section C - Gas chromatograph (GC), Line 3 (Column thermostat: operating temperature range no higher than (ambient T + 4°C) to 450°C. Thermostat volume not less than 13 liters).A46. The thermostat capacity parameter refers to the gas chromatograph column oven volume, which is already specified in Section C – Gas Chromatograph (GC).Since this parameter is not applicable to the flame ionization detector (FID) and duplicates the GC oven specification, the requirement will be removed from the FID section.

New clarification added: Q45.As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 19 (Heating rate: from no more than –250 to no less than +250 °C/min), please kindly remove this requirement as it contradicts Section C - Gas chromatograph (GC), Line 4 (Heating rate: not less than 120°C/min. Number of temperature programming stages: not less than 20).A45. The heating rate parameter refers to the gas chromatograph oven temperature programming capability, which is already defined in Section C – Gas Chromatograph (GC).Since this parameter is not applicable to the flame ionization detector (FID) and duplicates the oven specification, the requirement will be removed from the FID section.

New clarification added: Q44.As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 16 (Availability of a headspace (HS) auto-dispenser or equivalent system) please clarify whether the GC system must include a headspace autosampler in addition to the liquid autosampler, as Section G - Components, spare parts, consumables specify only liquid autosampler.A44. The gas chromatograph system under this procurement is required to be supplied with a liquid autosampler as specified in Section G – Components, spare parts, consumables.The availability of a headspace autosampler is not a mandatory requirement under the scope of this procurement.Therefore, the GC system must be equipped with a liquid autosampler, while the provision of a headspace autosampler is not required.

New clarification added: Q43. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 13 (Mass range: up to at least 1000 amu), please kindly remove this requirement as this parameter is not applicable to the FID characteristics.A43. The mass range parameter refers to the operational capability of a mass spectrometric detector (MSD) and is not applicable to the characteristics of a flame ionization detector (FID).Therefore, the requirement will be removed from the FID specification.

New clarification added: Q42.As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section C - Gas chromatograph (GC), Line 3 (Column thermostat: operating temperature range no higher than (ambient T + 4°C) to 450°C. Thermostat volume not less than 13 liters.), please kindly chage it to 'Column thermostat: operating temperature range no higher than (ambient T + 4°C) to 450°C. Thermostat volume not less than 12,5 liters' to encourage more paticipation.A42. The column oven volume defines the internal capacity of the chromatograph thermostat and ensures proper accommodation of capillary columns and accessories required for gas chromatographic analysis.To allow broader participation of suitable GC systems while maintaining adequate operational capability, the specification will be revised.Original requirementThermostat volume ≥13 L.Revised requirementThermostat volume ≥12 L.

New clarification added: Q41. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #4 HPLC with DAD and FLD detectors, Section I - Components, spare parts, consumables, Line 64 (Syringes for autosamplers - 2 pcs.) please kindly change it to 'Syringes for autosamplers - 2 pcs. (if a syringe-based dosing system is present)' to encourage more participation.A41. Autosampler designs differ among manufacturers, using either syringe-based, loop-based, or flow-through injection mechanisms, the latter not requiring dedicated syringes.To facilitate broader participation of suitable LC-MS/MS systems while maintaining the required autosampler functionality, the specification is being clarified:Original Requirement: Syringes for autosamplers – 2 pcs.Revised Requirement: Syringes for autosamplers – 2 pcs. (if a syringe-based injection system is used).

New clarification added: Q40. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #4 HPLC with DAD and FLD detectors, Section B - Technical Specifications, Line 47 (Sensitivity, Raman signal:noise :not less than 900) please kindly change it to 'Sensitivity, Raman signal:noise :not less than >550 ASTM ((>2100 using dark signal as noise reference)' to encourage more participation.A40. The Raman signal-to-noise parameter reflects the sensitivity of the diode array detector and is an important indicator of the detector’s ability to detect low absorbance signals and ensure reliable analytical performance.Different manufacturers may report detector sensitivity using different noise reference methodologies. To allow broader participation of suitable systems while maintaining adequate detector sensitivity, the specification will be clarified.Original requirementSensitivity, Raman signal: noise not less than 900.Clarified requirementSensitivity (Raman signal-to-noise ratio):≥900 when measured using the ASTM Raman signal-to-noise method,or equivalent detector sensitivity when specified using alternative manufacturer noise reference methods (e.g., dark signal noise reference or RMS noise method).

New clarification added: Q39.As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #4 HPLC with DAD and FLD detectors, Section B - Technical Specifications, Line 26 (Temperature stability is no more than 0.5°C.), please kindly change it to 'Temperature stability is no more than ±1˚C' to encourage more participation.A39. To allow broader participation of suitable HPLC systems, the specification will be revised.Original requirementTemperature stability ≤0.5°C.Revised requirementTemperature stability ≤±1°C.

New clarification added: Q38. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #4 HPLC with DAD and FLD detectors, Section B - Technical Specifications, Line 15 (Dosing range from 0.1 µl with the possibility of expansion to ≥2000 µl), please kindly change it to 'Dosing range from 0.1 µl with the possibility of expansion to ≥1000 µl' to encourage more participation.A38. The dosing range of the autosampler defines the operational flexibility of the system for different analytical methods and sample volumes.To allow broader participation of suitable HPLC systems while maintaining adequate analytical capabilities, the specification will be revised.Original requirementDosing range from 0.1 µl with the possibility of expansion to ≥2000 µl.Revised requirementDosing range from 0.1 µl with the possibility of expansion to ≥1000 µl.

New clarification added: Q37. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section I - Components, spare parts, consumables, Line 51 (Syringes for autosamplers - 2 pcs.), please kindly change it to 'Syringes for autosamplers - 2 pcs. (if a syringe-based dosing system is present)' to encourage more participation.A37. Different autosampler designs may use syringe-based or alternative injection mechanisms.Therefore, the requirement is clarified as follows:Revised requirement:Syringes for autosamplers – 2 pcs (if a syringe-based injection system is used).

New clarification added: Q36. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section G - Mass spectrometer, Line 2' (Mass registration stability is no worse than 0.05 amu /24 h), please kindly change it to 'Mass registration stability is no worse than 0.1 amu /24 h' to encourage more participation.A36. Mass registration stability reflects the stability of the mass scale during continuous operation.To allow broader participation of suitable LC-MS/MS systems while maintaining analytical performance, the specification will be revised.Original requirement:Mass registration stability ≤0.05 amu / 24 h.Revised requirement:Mass registration stability ≤0.1 amu / 24 h.

New clarification added: Q35. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section G - Mass spectrometer, Line 22 (Mass accuracy is no worse than ±0.1 m/z), please kindly exclude this requirement as the mass accuracy is determined by calibration and the relevant performance requirement is already covered in Item 24.A35. Mass accuracy represents an important performance parameter of the mass spectrometer.Therefore, the requirement remains unchanged.

New clarification added: Q34. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section E - Autosampler, Line 12 (Temperature control of samples - in the range of not less than 4–45 °C), please kindly change it to 'Temperature control of samples - in the range of not less than 4–40 °C' to encourage more participation.A34. To allow broader participation of suitable LC-MS/MS systems, the specification will be revised.Original requirement4–45°C.Revised requirement4–40°C.

New clarification added: Q33. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #2 Ion chromatography system, Section D - Column thermostat, Line 18 (Temperature range - Environment +5℃~50℃), please kindly change it to 'Ambient +7 °C, Setting range -15 to 60 °C' to encourage more participation and analytical methods.A33. The column thermostat temperature range establishes the operating conditions for chromatographic separation, ensuring stable and reproducible analytical performance. The specified range (Environment +5°C to 50°C) offers adequate operational flexibility for the intended applications and maintains stable temperature control of the column compartment. Since the proposed modification introduces different temperature reference conditions without offering a clear technical advantage for the intended analytical methods, the requirement remains unchanged.

New clarification added: Q32. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #2 Ion chromatography system, Section A - General Description, Line 1 (The device is designed for the separation, identification, and quantification of anions, cations, and polar molecules in liquid samples. The ion chromatograph provides highly sensitive analysis of complex mixtures and is used in laboratories for quality control and regulatory compliance.), please clarify whether a single-channel or a dual-channel ion chromatography system is required, as the specified analytical task, end-user needs to determine both anions and cations.A32. A dual-channel ion chromatography system capable of simultaneous determination of anions and cations is required.

New clarification added: Q31.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical specifications, Line 42 (Resolution: At least 15,000 (at m/z 1,522) and 8,000 (at m/z 118)), please kindly change it to 'Resolution: At least 40,000 (at m/z 200)', as the current resolution requirement is considered insufficient for reliable identification of antibiotics.A31. Mass resolution defines the ability of the mass spectrometer to distinguish ions with very small mass differences and is important for reliable identification of analytes and separation of isotopic peaks.To ensure reliable identification of compounds in complex food matrices and allow broader participation of suitable high-resolution LC-MS systems, the specification will be revised.Original requirementResolution ≥15,000 (at m/z 1522) and ≥8,000 (at m/z 118).Revised requirementResolution ≥30,000 (at m/z 200).

New clarification added: Q30.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical specifications, Line 40 (Mass range: At least 20–30,000 m/z.), please kindly change it to 'Mass range: At least 40–2500 m/z', as the current range is unnecessarily large for the target compounds and may limit participation of other suitable analytical systems.A30. The specified mass range defines the operational capability of the mass spectrometer. Considering the intended analytical applications for determination of antibiotics and dyes in food matrices, the required mass range may be adjusted without affecting analytical performance.To allow broader participation of suitable LC-MS systems, the specification will be revised.Original requirementMass range 20–30,000 m/z.Revised requirementMass range 40–2500 m/z.

New clarification added: Q29. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical specifications, Line 38 (Maximum scanning frequency: At least 30 Hz), please kindly change it to 'Maximum scanning frequency: At least 20 Hz', to avoid restricting the specification to one particular instrument model and to allow equipment from different manufacturers to participate.A29. Scanning frequency determines the number of spectra acquired across chromatographic peaks and influences quantitative accuracy.To allow broader participation of suitable LC-MS systems while maintaining adequate analytical performance, the specification will be revised.Original requirementMaximum scanning frequency ≥ 30 Hz.Revised requirementMaximum scanning frequency ≥ 20 Hz.

New clarification added: Q28. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical specifications, Line 28 (Injection cycle time: ≤ 30 seconds), please kindly change it to 'Injection cycle time: ≤ 10 seconds', as the original requirement is slow.A28. Injection cycle time defines the interval required for sample loading, injection, and preparation for the next analytical run.The specified value ensures reliable system operation and injection precision.Therefore, the requirement remains unchanged.

New clarification added: Q27. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical specifications, Line 29 ("Q-TOF High-Resolution Mass Spectrometer), we kindly ask to change this requirement to 'Q-TOF High-Resolution Mass Spectrometer/or High-Resolution Mass Spectrometer', because of the Insufficient resolution, difficulty analyzing multiply charged ions, poor separation of isotopic peaks, high sensitivity to temperature changes, frequent recalibration and background noise.A27. The system is intended for high-resolution mass spectrometric analysis of antibiotics and dyes in food matrices using liquid chromatography coupled with accurate mass detection.To allow broader participation of suitable high-resolution LC-MS systems while maintaining the required analytical capabilities, the specification will be revised.Original requirementQ-TOF High-Resolution Mass Spectrometer.Revised requirementQ-TOF High-Resolution Mass Spectrometer or High-Resolution Mass Spectrometer.

New clarification added: Q26.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section B - Technical specifications, Line 15 (Flow accuracy: 0.075% RSD.), we kindly ask to amend the flow accuracy ±0.1% as it is more indicative.A26. Flow accuracy reflects the stability and precision of solvent delivery in the chromatographic system and is important for maintaining reproducible retention times and reliable quantitative analytical performance.To allow broader participation of suitable LC systems while maintaining adequate analytical performance, the specification will be revised.Original requirementFlow accuracy: 0.075% RSDRevised requirementFlow accuracy: ±0.1%.

New clarification added: Q25. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #1 Liquid chromatograph with MSMS-TOF detectors, Section A - General Description, Line 1 (LC-MS/MS-TOF Automatic System. Used for separating compounds on a stationary phase column combined with TOF (Time-of-Flight) tandem mass spectrometry. Designed for determining and developing methods for antibiotic and dye content in food raw materials and food products), we kindly ask you to amend it with the following: “LC-MS/MS-TOF or LC MSMS with High Resolution Automatic System. Used for separating compounds on a stationary phase column combined with Q-TOF High-Resolution Mass Spectrometer/or High-Resolution Mass Spectrometer. Designed for determining and developing methods for antibiotic and dye content in food raw materials and food products", because of insufficient resolution, complex ion formation in antibiotics, limited dynamic range of TOF detectors, difficulty separating isotopic peaks, temperature sensitivity and frequent recalibration requirements that the original requirement implies.A25. The system is intended for the determination and development of analytical methods for antibiotics and dyes in food raw materials and food products using high-performance liquid chromatography coupled with high-resolution mass spectrometry.To ensure adequate analytical performance while allowing broader participation of suitable high-resolution LC-MS systems, the specification will be revised.Original requirement:LC-MS/MS-TOF Automatic System.Revised requirement:LC-MS/MS-TOF or LC-MS/MS with High-Resolution Mass Spectrometer Automatic System.All other technical requirements of the specification remain unchanged.  

New clarification added: Q24. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #5 GC with FID and MSD, Section E - Flame Ionization Detector (FID), Line 27 (Carrier gas flow rate through the injector: from 0 to at least 1300 ml/min), please increase the gas flow rate through the injector to 1000 ml/min, as the carrier gas flow rate typically does not exceed 5–7 ml/min and this requirement might limit competition.A24. The parameter refers to the maximum carrier gas flow capability of the injector system. While typical carrier gas flow rates used in capillary gas chromatography are significantly lower, the parameter defines the operational range supported by the injector.To allow broader participation of suitable GC systems while maintaining required functionality, the specification will be revised.Original requirement:Carrier gas flow rate through the injector: from 0 to at least 1300 ml/min.Revised requirement:Carrier gas flow rate through the injector: from 0 to at least 1000 ml/min.

New clarification added: Q23.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #4 HPLC with DAD and FLD detectors, Section B - Technical Specifications, Line 45 (The wavelength accuracy is no more than ±2 nm), please kindly increase the wavelength accuracy to ±3.0 nm, remains consistent with commonly accepted technical standards and ensures reliable performance for the intended application, as only a few models can achieve this value in the market, which my limit the competition.A23. Wavelength accuracy is the precision of a detector's ability to set and hold the selected wavelength for UV-visible measurements. This precise control is crucial for reliable identification, quantification, and consistent analytical results. Therefore, the requirement remains unchanged.

New clarification added: Q22. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #4 HPLC with DAD and FLD detectors, Section B - Technical Specifications, Line 20 (Cross-contamination level is no more than 0.0025% with caffeine solution.), please kindly increase the cross-contamination level to 0.005, which remains fully compliant with international standards and ensures safe and reliable performance, as only a few models can achieve this value in the market, which my limit the competition.A22. The cross-contamination parameter reflects the carry-over performance of the autosampler system. Achieving a low carry-over is essential for sequential and trace-level analysis, as it prevents contamination of subsequent samples and ensures reliable quantitative results. Consequently, the specified requirement remains unchanged, and compliance with this parameter will be mandatory for bid evaluation.

New clarification added: Q21.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section G - Mass spectrometer, Line 24 (Mass registration stability is no worse than 0.05 amu /24 h), please kindly increase the mass registration stability to 0.1 amu/24h, which remains suitable for the intended analytical use, as only few models can meet this requirement in the market, which may limit competition.A21. Mass registration stability reflects the long-term stability of the instrument mass scale during continuous operation and is important to ensure consistent identification of analytes and reproducibility of analytical results over extended analytical sequences.Considering the intended analytical applications and the fact that LC-MS/MS systems operate with unit mass resolution, a minor increase in the allowable mass registration drift does not significantly affect analytical performance.To ensure adequate analytical performance while allowing broader participation of suitable LC-MS/MS systems, the specification will be revised.The specification in Line 24 is amended as follows:Original requirement:Mass registration stability ≤ 0.05 amu / 24 h.Revised requirement:Mass registration stability ≤ 0.1 amu / 24 h.

New clarification added: Q20. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #3 Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Section G - Mass spectrometer, Line 20 ("Ionization source:- electrospray (ESI) with the ability to operate in positive and negative modes;- Polarity switching time is no more than 5 ms")), please kindly increase the polarity switching speed to 30, as only few models in the market are able to achieve this switching speed, which may limit competition.A20. The polarity switching parameter defines the speed at which the instrument alternates between positive and negative ion detection during analysis. This capability is important when analyzing compounds that ionize in different modes within the same analytical method.To ensure adequate analytical performance while allowing broader participation of suitable LC-MS/MS systems, the specification will be revised.The specification in Line 20 is amended as follows:Original requirement:Polarity switching time ≤ 5 ms.Revised requirement:Polarity switching time ≤ 30 ms.

New clarification added: Q19.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot # 1 (Liquid chromatograph with MSMS-TOF detectors), Section B - Technical Specifications, Line 48 (Recording of 200 spectra per scan time (2000 spectra/sec).), please kindly reduce this parameter to 50, as only a few models in the market are able to achieve this scanning speed, which may limit competition.A19. The parameter refers to the data acquisition speed of the mass spectrometer, which determines the number of spectra collected across chromatographic peaks and therefore influences quantitative accuracy in fast chromatographic separations.To ensure adequate analytical performance while allowing broader participation of suitable high-resolution LC-MS systems, the requirement will be revised.The specification in Line 48 is amended as follows:Original requirement:Recording of 200 spectra per scan time (2000 spectra/sec).Revised requirement:Recording of at least 20 spectra per second at a resolution setting of 15,000.All other requirements of the specification remain unchanged.

New clarification added: Q18. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot # 1 (Liquid chromatograph with MSMS-TOF detectors), Section B, Line 46 (Mass measurement accuracy (MS/MS mode): Better than 1 ppm.), please kindly consider to increase this parameter to 2 ppm as only a limited number of models currently available on the market achieve this level of mass measurement accuracy, which may potentially restrict competition.A18. The requirement “Mass measurement accuracy (MS/MS mode): Better than 1 ppm” defines the expected performance of the high-resolution LC-MS system for precise measurement of fragment ion masses.High mass accuracy in MS/MS mode is important for reliable compound identification, determining elemental composition, and confirming the structure of analytes, especially when analyzing complex samples or trace-level compounds.Maintaining this level of accuracy helps ensure consistent analytical results and reduces the risk of compound misidentification during high-resolution mass spectrometry analysis.Therefore, the requirement remains unchanged, and compliance with this parameter will be required during bid evaluation.

New clarification added: Q 17.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot # 1 (Liquid chromatograph with MSMS-TOF detectors), Section B - Technical Specifications, Line 34 (At least 4 integrated sample/syringe reservoirs for automated system setup/calibration controlled by software), please clarify whether the use of only two calibration solutions, as provided by some manufacturers’ equipment, would be considered a limitation under the specified requirements?A17. This requirement reflects the desired level of automation and flexibility for system calibration and tuning. Multiple reservoirs enable the automated preparation of calibration mixtures and system setup without manual intervention.However, systems with fewer calibration reservoirs may still be acceptable, provided the proposed configuration supports automated calibration procedures controlled by the instrument software, ensuring reliable calibration and stable analytical performance in line with the manufacturer’s specifications

New clarification added: Q16.  As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot # 1 (Liquid chromatograph with MSMS-TOF detectors), Section B - Technical Specifications, Line 32 (Ion sources included as standard with switching time not more than 20 ms), please clarify this as ion sources are not switched during the analysis.A16. This requirement specifies the mass spectrometer's capability to switch ionization polarity or operating mode during analysis. Rapid switching between positive and negative ion modes is often necessary for certain analytical workflows. The requirement does not, however, imply the physical replacement of ion source hardware during a single analytical run.

New clarification added: Q15. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot # 1 (Liquid chromatograph with MSMS-TOF detectors), Section B - Technical Specifications, Line 31 (Ionization: Atmospheric Pressure Ionization (API) and Electrospray Ionization (ESI) with LC interface including source and spray devices.), please clarify if the requirement implies to a single interchangeable ionization source platform capable of operating in both API and ESI modes OR two physically separate ionization sources to be supplied?A15. The requirement concerns the system's ability to support both ionization techniques commonly used in LC-MS analysis. This capability can be met either through an interchangeable ionization source platform that operates in the required ionization modes or through separate ionization sources supplied with the system. Both configurations are acceptable, provided the proposed system fully supports the necessary analytical ionization techniques.

New clarification added: Q14.As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot # 1 (Liquid chromatograph with MSMS-TOF detectors), Section B - Technical Specifications, Line 18 (Total system bandwidth: Max 12 µL.), please kindly clarify the intended purpose and mandatory nature of the specified parameter, and confirm whether compliance with this parameter is mandatory for bid evaluation, or whether alternative manufacturer specifications may be considered acceptable?A14. The parameter “Total system bandwidth: Max 12 µL” refers to the chromatographic dispersion of the LC system. Low system bandwidth helps maintain narrow peaks and improves sensitivity and resolution when used with high-resolution mass spectrometry.If this parameter is not explicitly stated in the manufacturer’s documentation, bidders may provide technical documents demonstrating equivalent chromatographic performance suitable for high-resolution LC-MS analysis, or a manufacturer’s statement confirming that the system meets this requirement.

New clarification added: Q69. As per the clarification, dated March 05, 2026, the deadline is extended to March 30, 2026, please confirm it.A69. The deadline for tender submission has been extended to March 30, 2026. However, the deadline for clarifications remains unchanged.

New clarification added: Q68. Could you please update the deadline, which is extended to March 30, 2026 as mentioned in clarification #13?A68. The deadline for tender submission has been extended to March 30, 2026. However, the deadline for clarifications remains unchanged.

New amendment added #1: The Invitation to Bid (ITB) has been amended to extend the deadline for submission of bids to 30 March 2026 at 07:00 UTC.All other requirements, terms, and conditions of the ITB remain unchanged.

New clarification added: Q13. In order to allow enough time to properly prepare the required technical and commercial documentation, could you extend the deadline by two (2) weeks, until March 30, 2026?A13. The suggestion is accepted. The deadline will be extended for another 2 (two) weeks. However, the clarification period will remain the same.

New clarification added: Q12. Although not specified in the technical parameters, a computer is essential for the operation of the Ion Chromatography System, and a UPS is required to protect the equipment from potential damage caused by power outages or voltage fluctuations. Could you please clarify whether the computer and UPS will be provided by the end user, or whether they should be included in our bid submission?A12. "As per line 37 of the Technical Specifications, ""The supplier (if necessary) must complete the equipment (taking into account the specifics of the proposed model) with all the necessary parts, components, materials (the cost of which should be included in the tender offer) for assembly, installation and commissioning at the workplace. Completeness should ensure the full functioning of the declared equipment.""Accordingly, a fully compatible PC is required, as specified by the manufacturer; however, a UPS does not need to be included in the offer.

New clarification added: Q11. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot #2 Ion chromatography system, Section G - Components, spare parts, consumables, Line 33 (Operation manual, maintenance manual, manufacturer's passport, certificates of conformity and quality), please clarify if the certificates of conformity and quality refer to CE, ISO9001?A11. Certificates of quality and conformity refer to those stipulated in the tender requirements for the technology being offered.

New clarification added: Q10. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot#5 (GC with FID and MSD), Section E - Flame Ionization Detector (FID), Line 20 (Maximum total program time: not less than 9999 minutes), please confirm if 9999 minutes is mandatory OR standard programmable limits are acceptable?A10. Standard programmable limits offered by major manufacturers shall be considered acceptable, provided that analytical flexibility is not materially restricted. 

New clarification added: Q9. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot#5 (GC with FID and MSD), Section E - Flame Ionization Detector (FID), Line 19 (Heating rate: from no more than –250 to no less than +250 °C/min), please confirm if ±250 °C/min is mandatory ORstandard manufacturer ramp rates are acceptable?A9. The requirement represents a maximum performance envelope. Standard manufacturer ramp rates that meet routine analytical application needs shall be considered acceptable. 

New clarification added: Q8. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot#5 (GC with FID and MSD), Section E - Flame Ionization Detector (FID), Line 18 (Temperature programming: at least 32 stages, with programmable cooling option), please confirm if 32 stages is mandatory OR standard industry configurations are acceptable, which is 20 ramps/plateaus?A8. The requirement reflects programming flexibility. Systems offering standard manufacturer ramp and plateau configurations commonly available in commercial GC systems may be considered acceptable, provided that analytical functionality is not materially restricted.

New clarification added: Q7. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot#5 (GC with FID and MSD), Section E - Flame Ionization Detector (FID), Line 14 (Detection limit: y e more than \( 1.2\ cdot 10^{-12}\) gC /sec (for carbon)), please clarify the wording 'y e more than' and confirm if 1.2 × 10⁻¹² gC/sec is a strict maximum limit OR minor deviations within standard manufacturer specifications are acceptable?A7.The correct interpretation is as follows:Detection limit: not more than 1.2 × 10⁻¹² gC/sec (for carbon).Compliance shall be assessed based on the official manufacturer’s specifications.

New clarification added: Q6. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot#5 (GC with FID and MSD), Section E - Flame Ionization Detector (FID), Line 13 ( Mass range: up to at least 1000 amu), please confirm if this requirement is intended for the MSD, as mass range is applicable to an MSD rather than an FID?A6. The mass range requirement applies to the Mass Selective Detector (MSD).

New clarification added: Q5. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot# 4 (HPLC with DAD and FLD detectors), DAD section, Line 41 (The presence of a mobile phase leakage sensor), please confirm if this requirement applies to the overall HPLC system OR to the DAD module specifically?A5. The requirement applies to the overall HPLC system safety design. The leakage sensor does not need to be integrated specifically within the DAD module, provided that the system incorporates an appropriate mobile phase leakage detection mechanism.

New clarification added: Q4. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot# 2 (  Ion chromatography system), Section D - Column thermostat:, Line 17 (Resolution - ≤0.0020 nS/cm), please confirm if ≤0.0020 nS/cm is mandatory OR minor deviations within standard industry specifications are acceptable?A4. The stated value represents a target sensitivity level. Minor deviations that remain within standard industry specifications, are supported by manufacturer documentation, and do not materially affect analytical performance may be considered acceptable.

New clarification added: Q3. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot# 2 (  Ion chromatography system), Section B - Technical Specifications, Line 5 (Gradient accuracy is no more than 0.15%), please confirm if 0.15% is a strict mandatory requirement; OR ±0.5% (which represents the typical industry standard) is acceptable?A3. The requirement represents a target performance level. Systems offering gradient accuracy up to ±0.5%, supported by official manufacturer documentation and demonstrating comparable analytical reproducibility, may be considered acceptable.

New clarification added: Q2. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot#1 (Liquid chromatograph with MSMS-TOF detectors), Section D (Components, spare parts, consumables), Line 79 (High-purity oil for pump: not less than 5 liters), please confirm if the oil requirement applies only to oil-sealed pumps, OR oil-free vacuum systems are acceptable and the oil requirement may be considered not applicable in such cases, as a bidder proposed configuration uses an oil-free dry backing pump?A2. The requirement applies when an oil-sealed pump is provided. If the proposed system uses an oil-free dry backing pump that does not require oil for operation, the oil supply requirement may be considered Not Applicable, as long as all specified vacuum performance parameters are satisfied.

New clarification added: Q1. As per ITB_Section III Returnable Bidding Forms, Form D - Technical Bid Form, Lot#2 (Ion chromatography system), Section G, Line 33 (Operation manual, maintenance manual, manufacturer's passport, certificates of conformity and quality), please clarify what does manufacturer's passport refer to?A1.Please note that the manufacturer's passport includes the operation manual, maintenance manual, certificates of conformity and quality, the manufacturer's calibration report, and the warranty certificate.